In the fast-evolving field of biopharmaceuticals, CHO (Chinese Hamster Ovary) serum-free media has emerged as a game-changing solution for scalable, high-performance cell culture. Designed to eliminate reliance on animal-derived serum, this advanced media ensures consistent cell growth, enhances protein yield, and simplifies regulatory compliance—critical advantages for modern therapeutic development.
OptiVitro® CHO Serum-free Basal media series is a state-of-the-art, animal-free, protein-free, and chemicallydefined media that is specifically designed for the high-density culture of CHO-K1, CHO-DG44, and CHO-S etc.
Serum-free,no animal-derived components chemically defined
From early discovery to commercial scale production
Maintains high-density, high-viability growth of various CHO cell types
IS0 9001:2015 certified manufacturing
USP<1043>and EP Chapter 5.2.12 standards
Customized medium optimization
Customized manufacture (liguid/dry powder)
♦Improved cell viability and growth
♦Consistent and reliable results
♦Simplified downstream processing
♦Reduced batch-to-batch variability
♦Compliance with regulatory standards
OptiVitro® CHO Serum-free Medium TransExp CE06/CA06 serum-free, animal-derived component-free, chemically defined culture media. It is suitable for high-density culture and transient transfection protein expression of CHO-S, especially ExpiCHO-S cells.
| Product Name | Cat.# | Specification |
|---|---|---|
| OptiVitro® CHO Serum-free Basal Medium TransExp CE06 | CE000-N052 | 1L, liquid |
| CE000-N053 | 1L, powder | |
| CE000-N054 | 10L, powder | |
| CE000-N055 | 100L, powder | |
| OptiVitro® CHO Serum-free Feed Medium TransExp CA06 | CA000-N032 | 100mL, liquid |
| CA000-N033 | 1L, liquid | |
| CA000-N034 | 1L Powder | |
| CA000-N035 | 10L Powder | |
| OptiVitro® Glucose solution | M101382C | 100mL, liquid |
| OptiVitro® Protein expression enhancer | M101412C | 1mL, liquid |
| M101413C | 5mL Liquid |
♦Serum-free, no animal-derived components, chemically defined
♦Maintains high-density cell growth under optimized conditions
♦User-friendly workflow - Streamlined protocols minimize hands-on time and operational complexity
♦High-yield performance - Delivers superior protein expression levels (compatible with feed supplementation for further productivity boost)
Combining intuitive single-feed setup with yields exceeding 1g/L within 7 days, our platform redefines efficiency in transient protein expression
♦No animal-derived components, chemically defined
♦suitable for high-density culture of CHO-K1, CHO-DG44, CHO-S, and CHOZN cells, as well as high-level expression of recombinant proteins.
Application scenarios: Cryopreservation of CHO cells, seed expansion, batch culture, and fed-batch culture (used with feed culture medium).
♦Feed supplemented with CA01β
♦No animal-derived components, chemically defined
♦Suitable for CHO fed-batch culture processes, supplementing nutrients consumed during cell culture to improve protein yield and cell density
Application scenarios: Fed-batch culture (used with basal culture medium)
OptiVitro® CHO Serum-free Basic Culture Medium is a serum-free, animal-derived component-free, chemically defined cell culture medium suitable for Chinese hamster ovary cells (CHO) for recombinant protein expression. It is suitable for CHO cell cryopreservation, seed expansion, batch culture, and fed-batch culture (used with feed culture medium).
♦Serum-free, no animal-derived components, chemically defined
♦Maintains high-density, high-viability growth of various CHO cell types
♦Supports high-yield, high-quality protein production by CHO cells
♦Provides formula optimization and customization services
♦Support clinical application and commercialization
OptiVitro® CHO CA01α/02α/03α and CA01β are animal-derived component-free, chemically defined feed culture media suitable for CHO fed-batch culture processes. They supplement nutrients consumed during cell culture to improve protein yield and cell density.
♦Serum-free, no animal-derived components, chemically defined
♦Effectively improves cell density and viability in fed-batch culture
♦Supports high-yield, high-quality protein production by CHO cells
♦Provides formula optimization and customization services
♦Support clinical application and commercialization
ExCell provides ‘3×3’ combination which contains basal media and main feed media in OptiVitro® CHO Serum-Free Culture Media Test Kit.
| Combination | Basal | Main Feed | Supplement |
|---|---|---|---|
| 1 | CE01 | CA01α | CA01β |
| 2 | CE01 | CA02α | |
| 3 | CE01 | CA03α | |
| 4 | CE02 | CA01α | |
| 5 | CE02 | CA02α | |
| 6 | CE02 | CA03α | |
| 7 | CE03 | CA01α | |
| 8 | CE03 | CA02α | |
| 9 | CE03 | CA03α |
0.6–1.0 × 10⁶ cells/mL. Seed cells should be in the logarithmic growth phase with a viability of no less than 95%.
Temperature: 37°C
Humidity: 80% relative humidity
CO₂: 5%
120–150 rpm, 50mm (reduce speed appropriately for larger volumes).
If conditions allow, pass cells once or more in the three basal media prior to inoculation. Alternatively, direct inoculation into the test media can be conducted, in which case centrifugation and resuspension is recommended to minimize the influence of previous media on screening results.
| Day | Main Feed CE01 (%) | Supplement CA01β (%) (10% of Main Feed) |
|---|---|---|
| D03 | 3.0% | 0.30% |
| D05 | 5.0% | 0.50% |
| D07 | 5.0% | 0.50% |
| D09 | 5.0% | 0.50% |
| D11 | 4.0% | 0.40% |
| D13+ | If viability > 80%, feed every other day: 3–4% (0.3–0.4%). | |
Contact us for feeding strategies
OptiVitro® Cloning Medium is a serum-free, animal-derived component-free, chemically defined culture medium developed for single-cell cloning applications. It significantly improves the proliferation rate of single clones, maintains good cell status, and enables rapid screening of high-quality clones.
| Catalog | Specification | Shelf Life | Storage |
|---|---|---|---|
| UE000-N091 | 100 mL liquid | 12 months | 2-8°C, protect from light |
| UE000-N092 | 500 mL liquid |
♦No animal-derived components, chemically defined
♦Supports single-cloning of various CHO and 293 cell types
♦Significantly improves single-clone proliferation rate
♦Perfectly replaces cloning media containing hydrolysates
OptiVitro®CHO serum-free perfusion medium CP01: This medium does not contain any animal-derived components and has clearly defined chemical compositions. It is suitable for N-1 perfusion culture, steady-state culture, and other multi-purpose applications for CHO-K1, CHOZN, and CHO-S cells.
| product name | product number | specifications | storage | shelf life |
|---|---|---|---|---|
| OptiVitro® CHO Serum-free Perfusion Medium CP01 | CE000-N071 | 1000mL | 2-8℃ dark | 12 mouths |
| CE000-N072 | 1L(powder) | 2-8℃ dry、dark | 24 mouths | |
| CE000-N073 | 10L(powder) | |||
| CE000-N074 | 50L(powder) | |||
| CE000-N075 | 100L(powder) |
♦Contains no animal-derived components, with clearly defined chemical composition;
♦simplified formulation, no need for acid-base adjustment;
♦supports N-1 perfusion and steady-state culture: maintains high-density and high-viability growth of various types of CHO cells;
♦fully self-developed, stable supply;
♦can provide formula optimization and customized medium services.
Click on the product name in the table to go to the product details page.
| Basal Media | Feed Media | |
|---|---|---|
| Stable Transfection Media | OptiVitro® CHO Serum-free Basal Medium CE01 | OptiVitro® CHO Serum-free Feed Medium CA01α |
| OptiVitro® CHO Serum-free Basal Medium CE02 | OptiVitro® CHO Serum-free Feed Medium CA02α | |
| OptiVitro® CHO Serum-free Basal Medium CE03 | OptiVitro® CHO Serum-free Feed Medium CA03α | |
| \ | OptiVitro® CHO Serum-free Feed Medium CA01β | |
| Transient Transfection Media | OptiVitro® CHO Serum-free Basal Medium TransExp CE06 | OptiVitro® CHO Serum-free Feed Medium TransExp CA06 |
| Monoclonalization | OptiVitro® Serum-free Cloning Medium | \ |
| Perfusion Media | OptiVitro® CHO Serum-free Perfusion Medium CP01 | \ |
| Product Name | Specification | Catalog Number | |
|---|---|---|---|
| OptiVitro® CHO Serum-Free Culture Media Test Kit | 1 Kit | UE000-N111 | Recommend Test Combinations |
| Optivitro® Serum-free Cell Cryopreservation Medium UC04 | 100 mL | UC000-N056 | Recommended to be used with SFM |
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